Detecting of Some Klebsiella Pneumoniae Resistance Genes Isolated from Different Sources


  • Adian Abd Alrazak Dakl
  • Ikram Abbas Aboud Al –Samrraee



This research included 45 isolates of Klebsiella pneumoniae from out of 114 specimens  gathered  from different sources  from (animal and  human)  ; all isolates were diagnosed on Hi-Crome klebsiella Selective agar base and Hi-Crome ESBL Chromagar  which isolate just resistant Klebsiella pneumoniae  strain of  extended spectrum beta lactamase  antibiotic group. The discs method (Kirby-Bauer) was used to conduct the sensitivity test for resistant isolates to ascertain Klebsiella pneumoniae's resistance to 24 different antibiotics.  . The results investigated  that 21 Klebsiella pneumoniae isolates  from out of 45 isolates  was resistant by growth on ESBL Chromagar  and  the highest resistance was against Cefuroxime, Cefadroxil, Ceftriaxone,Co-Trimoxazole and Norflxacin, while Klebsiella pneumoniae  was  less resistant to Cefepime, Cefpirome and Aztreonam   and sensitive to Imipenem and Tobramycin,Pipracillin and Etrapenem .                                                                                                                                                   

Three Klebsiella pneumoniae isolates was diagnosed molecularly by using conventional  polymerase chain reaction, 16S rRNA and detection of some resistance genes (blaOXA48, qnrB, sul1 and  aac(6’)-Ib-cr ). Results showed that when PCR was carried out using the primer that targets the 16S rRNA, all isolates of  Klebsiella pneumoniae produced a distinct band with a molecular size of 1250 bp. When using the primer specific for the resistance genes  blaOXA48, qnrB, sul1 and  aac (6’) -Ib-cr, there are two isolates carry three out of  four  examined resistance genes which gave a band of 744 bp in size for the blaOXA48 gene,469 bp,436bp and 482 bp  to qnrB,sul1 and aac(6’)-Ib-cr  genes  respectively. These results suggest that blaOXA48, qnrB, sul1 and  aac (6’)-Ib-cr genes  might be a useful marker to identify resistant Klebsiella pneumoniae  strains.                                                                                     




How to Cite

Adian Abd Alrazak Dakl, & Ikram Abbas Aboud Al –Samrraee. (2023). Detecting of Some Klebsiella Pneumoniae Resistance Genes Isolated from Different Sources . Migration Letters, 20(S7), 334–340.