Detecting of Some Klebsiella Pneumoniae Resistance Genes Isolated from Different Sources
This research included 45 isolates of Klebsiella pneumoniae from out of 114 specimens gathered from different sources from (animal and human) ; all isolates were diagnosed on Hi-Crome klebsiella Selective agar base and Hi-Crome ESBL Chromagar which isolate just resistant Klebsiella pneumoniae strain of extended spectrum beta lactamase antibiotic group. The discs method (Kirby-Bauer) was used to conduct the sensitivity test for resistant isolates to ascertain Klebsiella pneumoniae's resistance to 24 different antibiotics. . The results investigated that 21 Klebsiella pneumoniae isolates from out of 45 isolates was resistant by growth on ESBL Chromagar and the highest resistance was against Cefuroxime, Cefadroxil, Ceftriaxone,Co-Trimoxazole and Norflxacin, while Klebsiella pneumoniae was less resistant to Cefepime, Cefpirome and Aztreonam and sensitive to Imipenem and Tobramycin,Pipracillin and Etrapenem .
Three Klebsiella pneumoniae isolates was diagnosed molecularly by using conventional polymerase chain reaction, 16S rRNA and detection of some resistance genes (blaOXA48, qnrB, sul1 and aac(6’)-Ib-cr ). Results showed that when PCR was carried out using the primer that targets the 16S rRNA, all isolates of Klebsiella pneumoniae produced a distinct band with a molecular size of 1250 bp. When using the primer specific for the resistance genes blaOXA48, qnrB, sul1 and aac (6’) -Ib-cr, there are two isolates carry three out of four examined resistance genes which gave a band of 744 bp in size for the blaOXA48 gene,469 bp,436bp and 482 bp to qnrB,sul1 and aac(6’)-Ib-cr genes respectively. These results suggest that blaOXA48, qnrB, sul1 and aac (6’)-Ib-cr genes might be a useful marker to identify resistant Klebsiella pneumoniae strains.
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