Detecting of Some Klebsiella Pneumoniae Resistance Genes Isolated from Different Sources

Abstract

This research included 45 isolates of Klebsiella pneumoniae from out of 114 specimens  gathered  from different sources  from (animal and  human)  ; all isolates were diagnosed on Hi-Crome klebsiella Selective agar base and Hi-Crome ESBL Chromagar  which isolate just resistant Klebsiella pneumoniae  strain of  extended spectrum beta lactamase  antibiotic group. The discs method (Kirby-Bauer) was used to conduct the sensitivity test for resistant isolates to ascertain Klebsiella pneumoniae's resistance to 24 different antibiotics.  . The results investigated  that 21 Klebsiella pneumoniae isolates  from out of 45 isolates  was resistant by growth on ESBL Chromagar  and  the highest resistance was against Cefuroxime, Cefadroxil, Ceftriaxone,Co-Trimoxazole and Norflxacin, while Klebsiella pneumoniae  was  less resistant to Cefepime, Cefpirome and Aztreonam   and sensitive to Imipenem and Tobramycin,Pipracillin and Etrapenem .                                                                                                                                                   

Three Klebsiella pneumoniae isolates was diagnosed molecularly by using conventional  polymerase chain reaction, 16S rRNA and detection of some resistance genes (blaOXA48, qnrB, sul1 and  aac(6’)-Ib-cr ). Results showed that when PCR was carried out using the primer that targets the 16S rRNA, all isolates of  Klebsiella pneumoniae produced a distinct band with a molecular size of 1250 bp. When using the primer specific for the resistance genes  blaOXA48, qnrB, sul1 and  aac (6’) -Ib-cr, there are two isolates carry three out of  four  examined resistance genes which gave a band of 744 bp in size for the blaOXA48 gene,469 bp,436bp and 482 bp  to qnrB,sul1 and aac(6’)-Ib-cr  genes  respectively. These results suggest that blaOXA48, qnrB, sul1 and  aac (6’)-Ib-cr genes  might be a useful marker to identify resistant Klebsiella pneumoniae  strains.