Preparation of A New Azo-Reagent Derivative from 8-hydroxy Quinoline for Determination of Copper (II) ion Using Spectrophotometric Technique

Abstract

A new, sensitive, and accurate spectrophotometric analytical technique is used to determine copper (II) ion in different samples using a new azo derivative reagent of 8-hydroxy quinoline;4-((8-Hydroxy quinolin5-yl) diazenyl) benzoic acid (HQDABA). To diagnose the reagent and Cu(II) complex, Infrared spectroscopy, Ultraviolet-Visual radiation, Elemental Analysis (CHN), and techniques such as nuclear magnetic resonance spectroscopy (1HNMR) and mass spectroscopy were used. The approach is based on the interaction of copper (II) with the HQDABA reagent in a neutral solution, which results in the formation of a yellow complex with a maximum absorbance at 420 nm. The optimal circumstances, such as medium pH, reagent volume, reagent concentration, and time effect, were also thoroughly examined. The limits of detection (LOD), limits of quantification (LOQ), and Sandell sensitivity were determined to be 0.22 mgL-1, 0.740 mgL-1, and 7.46210-2g.cm-2, respectively. The proposed approach complied with beer's low concentration range of 0.5-15 mgL-1, and the recovery percentage of the various samples ranged from 78.26% to 111.53%.  The proposed spectrophotometric technique has been shown to be simple, quick, and sensitive for determining Cu (II) ions in various materials.