Isolation and identification of opportunistic fungi that causes for Otitis

Abstract

The purpose of this study was to isolate and characterize Aspergillus niger. Isolated from patients with otitis media using various identification techniques, such as B. direct examination and laboratory culture. Used to observe yeast hyphae, large and small spores and chlamydospores . A total of 30 swabs were taken from patients with otitis throughout the months of June through November 2022 while they were receiving care at AL- Sadder Medical City (specialist ENT). The samples were taken as 30 ear swabs from otitis patients in AL-Najaf Governorate. The findings of this study indicate that Aspergillus niger and other opportunistic fungus caused more cases of otitis than Aspergillus sp.. The findings of the current study show that Aspergillus niger was more common in otitis patients than Candida albicans, with prevalence rates of 17 (77.27%) and 5 (22.72%), respectively. The results show the presence of 22 isolates belonging to the genera Aspergillus niger & Candida albicans, This phenomenon was observed in both female and male patients after culturing ear specimens on the primary separation medium Sabouraud dextrose agar (SDA) and secondary medium on additional potatokdextrose agar (PDA).